Determination of lipid content in adipocyte cells
Determination of lipid content (Reed & Lane, 1980) was modified
as follows. Adipocyte cells grown on a 12-well plate were
washed twice with 1 mL of PBS and were incubated with 1 mL of
10% formalin for 10 min. After the incubation, the cells were
washed twice with 1 mL of PBS and were incubated with 1 mL of
60% isopropanol for 1 min. After the removal of 60% isopropanol,
cells were stained with 1 mL 0.18% Oil Red O/60% isopropanol for