The skins and swim bladders (0.125 kg) were soaked in 0.1 M NaOH for 36 h, with the alkaline solution being changed every 12 h. During alkaline treatment, the skins and swim bladders would swell, and a sample/alkaline solution ratio of 1:30 (w/v) was used to ensure the mixture could be stirred by the magnetic stirrer and a sample/butyl alcohol solution ratio of 1:30 (w/v) was also used during the following defatting treatment. The deproteinised residues were suspended in 10% (v/v) butyl alcohol for 36 h with a change of solution every 12 h. The defatted residues were thoroughly washed with cold distilled water, and then suspended in 0.5 Macetic acid containing 0.1% (w/v) pepsin for 3 days. During the extracting process, the viscosity of the solution would greatly increase, and a sample/solution ratio of 1:40 (w/v) was used to ensure the solution stay with the appropriate viscosity and can be stirred by the magnetic stirrer. The subsequent procedures were performed essentially as those applied to the fins and bones.
The skins and swim bladders (0.125 kg) were soaked in 0.1 M NaOH for 36 h, with the alkaline solution being changed every 12 h. During alkaline treatment, the skins and swim bladders would swell, and a sample/alkaline solution ratio of 1:30 (w/v) was used to ensure the mixture could be stirred by the magnetic stirrer and a sample/butyl alcohol solution ratio of 1:30 (w/v) was also used during the following defatting treatment. The deproteinised residues were suspended in 10% (v/v) butyl alcohol for 36 h with a change of solution every 12 h. The defatted residues were thoroughly washed with cold distilled water, and then suspended in 0.5 Macetic acid containing 0.1% (w/v) pepsin for 3 days. During the extracting process, the viscosity of the solution would greatly increase, and a sample/solution ratio of 1:40 (w/v) was used to ensure the solution stay with the appropriate viscosity and can be stirred by the magnetic stirrer. The subsequent procedures were performed essentially as those applied to the fins and bones.
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