The phage formulation was applied on the infected skin lesion of ten C. gariepinus with sterile cotton swab.
On the contrary, infection control fish with similar sized infected skin lesion were applied with phage free diluents to compare with infected fishes undergoing
phage therapy.
The phage free diluent was applied to infection control to avoid the effect of a phage diluent placebo.
The water temperature of the fish tanks was 20 ± 1°C throughout the experiments.
Statistical analysis for phage treatment Statistical tool like Minitab 16 software free trial version
was applied to perform the statistical analysis of bacteriophage
treatment on infected Clarias gariepinus