Dense inoculants of selected strains were inoculated into test tubes containing 10ml of Brain Heart infusion broth with pH of 5.8; the inoculated media were incubated at 280C for 7 days with constant agitation. At the end of the incubation, the cultures were washed by centrifugation at 4000g for 30mins (Kanlayani et al) after which the crude supermatant were assayed using the Agar well diffusion technique. The entire surface of Mueller-Hinton plate was inoculated via spread plate technique using sterile Swab stick, sterile precipitin tubes were used to bore the media and 0.5ml portion of the crude supermatant was immediately introduced into the well and then incubated at 370C for
24hours