Two new species of Anchylodiscus Johnston & Tiegs, 1922, A. malayensis n. sp. and A. liewi n. sp., were obtained from the gills and the dendritic (or arborescent) organ of Plotosus canius, respectively. The two new species have two seminal vesicles; the seminal vesicle nearer the testis is a distension of the vas deferens, while the distal one is similar to the blind seminal vesicle of the majority of freshwater ancylodiscoidines. The blind seminal vesicle suggests that Anchylodiscus should be placed in the subfamily Ancylodiscoidinae.