Biscuit texture, dimensions, and weight loss during baking were strongly related to microstructure of doughs and biscuits. In high fat content doughs (0, 10, and 20 % fat replacement levels) fat was observed surrounding flour components. This ingredient distribution hindered flour hydration and promoted the lubrication of the structure. These effects gave doughs with soft texture. During baking, fat melted and coated the matrix surface. This fact restricted flour hydration, and so water loss during baking increased. Moreover, high fat content improved biscuit expansion and aeration. Biscuit texture was highly influenced by its microstructure; biscuits with high fat content were brittle and soft, in contrast to dough with higher fat replacement (30 %) where the absence of fat enables a higher hydration of the component, giving harder biscuits. However, when fat replacement by inulin was 40 %, there was not enough fat to lubricate the system; thus, a fragile structure was obtained. Biscuits with 20 % of fat replacement had similar structural and textural behavior than its counterpart, although in the sensory evaluation the