The assay was performed according to the methodology
described by Singleton and Rossi (1965). The extracts (0.25 mL)
were mixed with 0.25 mL of Folin–Ciocalteu reagent, 2.5 mL of
ultrapure water and 0.25 mL of sodium carbonate (10%). They were
kept at room temperature for 60 min, with absorbance reading at
725 nm using the UV/Vis V-630 spectrophotometer (Jasco, Tokyo,
Japan). The results were expressed in milligrams of GAE (gallic acid
equivalent) per 100 g of fresh sample.