Automated pumping of bicarbonate, mineral medium
and purge was performed to warrant the continuous
renewal of the liquid phase. Mineral medium contained (g L1):
NH4Cl, 1; KH2PO4, 0.12; K2HPO4, 0.15; CaCl2, 0.02; MgSO4.7H2O,
0.2; and trace elements [19], 1 mL L1. During the present experiments,
a trickling liquid velocity (TLV) of 7 m h1 and a hydraulic
retention time (HRT) of 9 h were maintained.