PCR
amplification was performed in a Gene System 9600 Thermocycler
(Perkin Elmer, USA). A 30 l PCR reaction mixture was comprised
of 100 ng genomic DNA, 1.5 U Taq polymerase (Promega), 1 mM
MgCl2 and 0.5 M mixed CHS gene primer set. The following CHS
primer sequence sets were used: CHS F: 5' –ATGGTGACAG
TCGAGGAG-3') and CHS R: 5'-TTAAGTAGCAACACTGTG-3'.