FVIII expression in WT mice
hFVIII:Ag was undetectable in WT mice before Dox adminis- tration. Dox administration began at 1-week post- transplantation, and hFVIII:Ag was detected in the plasma from three of the six transplanted mice (0.4–1.5% hFVIII: Ag of the normal hFVIII:Ag level) on Day 10. Although hFVIII:Ag became undetectable in one mouse, two mice continued to produce FVIII on Day 20. One mouse died on Day 20 after blood collection, but the other mouse produced
FVIII until Day 30 (Fig. 2B). CCl4-induced liver injury did not cause fatal liver failure in WT mice. Tumor formation was not observed by laparotomy in transplanted mouse spleens or the livers.