Biotransformation assays
Biotransformation of HCB and its dechlorination
products was studied under both simulated in situ
conditions and conditions intended to enhance native
microbial activity and/or increase the bioavailability of the
sediment contaminants for biotransformation. Chlorinated
benzenes were not added to the microcosms in any of the
biotransformation assays, but rather the field contaminated
sediment samples were the source of the contaminants. To
perform these studies, two assays were carried out in 21
glass bottles (1800ml liquid volume). Incubation was
carried out in the dark at 23°C. Periodically, the bottles were
manually shaken. Continuous mixing was avoided to
prevent breaking up of the sediment particles.