A 10% inoculum was used in the 500-ml baffled Erlynmeyer flasks for submerged fermentation.
The modified mineral medium
(actinorhodin mineral medium (AMM)) was prepared as gram per liter of distilled water containing 9.0 NaNO3; 0.3 K2HPO4;
2.0 FeSO4; 1.6 MnSO4; 0.04 ZnSO4; 0.2 CoCl2; 0.1 MgSO4; 0.05 CaCl2; 2.0 NaCl; 1% sponge extract; 0.001 cAMP.