Although sorghum tissue culture has been studied formany decades,
a highly efficient and comprehensive system has remained elusive till
date. Poor long-term callus regenerability is considered as one of the
major obstacles in sorghum tissue culture (Raghuwanshi and Birch,
2010). Sorghum inbred line SA281, which regenerated embryogenic callus
well but produced a lot of phenolics in previous experiments, has
been utilized to study sorghum tissue culture in our laboratory for many
years. So we started with SA281 to optimize medium and subsequently
used another two lines (Tx430 and 91419R) in our tissue culture system.
Hence, the aim was to define a robust tissue culture system for sorghum
by optimizing media and investigating the effect of callus age on
regenerability.