Determination of MIC was performed using the broth microdilution
method, following the norm M38-A of the National Committee
for Clinical Laboratory Standard (NCCLS, 2002). TEO was diluted
in a sterile solution of Tween 80 at 0.001% (Vetec, Rio de Janeiro,
Brazil) and evaluated in final concentrations that ranged from
6.25 to 4000 lg/mL.