Assay for siderophore production
According to the methodology described by Gopalakrishnan [10],
bacteria were streaked on the center of Chrome Azurol S (CAS) agar
media and incubated at 28 ± 2°C for 48h. When the bacteria consume
iron, present in the blue-colored CAS media, orange halos around the
colonies indicate the incidence of siderophores.