Hyperbaric oxygen treatment consisted of placing
animals inside a hyperbaric treatment chamber where they
received 100% oxygen at a pressure of 2.4 ATA. During each
treatment animals were slowly brought from room pressure to
depth (5–7 min), they received a 90 min treatment, and were then
resurfaced to normal room pressure again (5–7 min). This protocol
was chosen as it reflects a typical clinical treatment regimen for
chronic wounds treated in a monoplace chamber. Therefore, each
treatment lasted in total approximately 100–104 min. The control
group was simply placed inside the hyperbaric treatment chamber
for approximately 100 min, and did not receive any treatment.