Example micrograph. A, The average of 30 frames after least squares alignment of whole frames for an exposure series movie of the Saccharomyces cerevisiae V-ATPase
in ice. The exposure used 2.5 e/pixel/frame and each pixel corresponds to 1.45 1.45 Å. The S. cerevisiae V-ATPase has a molecular weight of 900 kDa. Several example
complexes are circled in red. B, An individual frame from the movie shows the low SNR in frames.