For DNA extraction, we used 100 mg leaves from each sample in all protocols, with
the exception of the Mogg and Bond (2003) protocol where 1 cm2 leaves was used. The samples
were ground in liquid nitrogen immediately before the procedure. The equipment and
materials used in all protocols were: 1) mortar and pestle; 2) 1.5- and 2.0-mL microcentrifuge
tubes; 3) liquid nitrogen; 4) water bath (65°C), and 5) centrifuge and rotor capable of 14,000
rpm (17,746 g). Below are the brief explanations of protocols A, B, and C, and a detailed explanation of protocol D.