Figure 2. Western blot reaction of the serum samples from
patients HuC1 and HuC2 from Italy showing allergic reaction
against Anisakis pegreffii antigens and allergens. M indicates
molecular marker; arrow indicates the reaction at 24 kDa (Ani
s1). IgE determination was performed with alkaline phosphatase
conjugates obtained from goat anti-human IgE. Antigen-antibody
binding was visualized by the alkaline phosphatase 5-bromo-4-
chloro-3-indolyl phosphate p-nitroblue tetrazolium chloride system
until bands appeared. Human serum specimens negative for A.
pegreffii were used as controls. HC1 and HC2 represent patient
identification numbers HuC1 and HuC2.