EVA again proved to be the most effective polymer in the active packaging.
EVA films (control and spiked with 3% and 6% PVPP-WS extract)
were tested againstmicroorganisms that aremost susceptible to the natural
extract (S. aureus CECT 239 and E. coli ATCC 8739) (Table 3). The EVA
film spiked with the lower concentration of extract (EVA3%) was active
against S. auerus after 48 h of contact. EVA6% was effective against both
microorganisms tested during long incubation times for E. coli and
throughout the entire incubation time for S. aureus. All EVANANO active
films showed antimicrobial activity against S. aureus during incubation.
Inclusion of the functionalized nanoclays improved the effectiveness of
the active packaging film, even though they contained lower amounts
of natural extract.