4. Conclusions
Argentine molasses and grapes were used to isolate 29 ethanolproducing yeasts,finding three isolates with higher ability to produce ethanol. One of these isolates showed a homogeneous growth
(A2) meanwhile the other two presented aflocculent growth (A10
and A11) when they were grown in commercial media. It was also
observed that the three isolates presented a percentage of similarity of 100% with the strain S. cerevisiae. The three strains were
highly sensitive to changes in temperature,finding an optimal
temperature for the production of ethanol at 30
C, and although
they could tolerate up to 300 g/L without decreasing the production
of ethanol, the best results were achieved using 250 g/L of initial
sugar. Higher sugars concentrations (350 g/L) represented a
decrease in ethanol concentration, particularly using the nonflocculent strain A2, thus concluding thatflocculation can act as a
protective mechanism in yeast stress conditions such as with high
concentration of sugars, and consequently theflocculent characteristics of strains A10 and A11 protected a high osmotic pressure
generated by high sugar concentration. These strains were also able
to grow in molasses medium although the behavior of the nonflocculent A2 strain was faster, probably because of its dispersed
form facilitates the transfer of nutrients and products. The process
can be successfully scaled up from laboratory scale to 10L bioreactor, thus demonstrating the feasibility of theS. cerevisiaeA2 strain
to be used as a novel strain to produce ethanol
4. ConclusionsArgentine molasses and grapes were used to isolate 29 ethanolproducing yeasts,finding three isolates with higher ability to produce ethanol. One of these isolates showed a homogeneous growth(A2) meanwhile the other two presented aflocculent growth (A10and A11) when they were grown in commercial media. It was alsoobserved that the three isolates presented a percentage of similarity of 100% with the strain S. cerevisiae. The three strains werehighly sensitive to changes in temperature,finding an optimaltemperature for the production of ethanol at 30C, and althoughthey could tolerate up to 300 g/L without decreasing the productionof ethanol, the best results were achieved using 250 g/L of initialsugar. Higher sugars concentrations (350 g/L) represented adecrease in ethanol concentration, particularly using the nonflocculent strain A2, thus concluding thatflocculation can act as aprotective mechanism in yeast stress conditions such as with highconcentration of sugars, and consequently theflocculent characteristics of strains A10 and A11 protected a high osmotic pressuregenerated by high sugar concentration. These strains were also ableto grow in molasses medium although the behavior of the nonflocculent A2 strain was faster, probably because of its dispersedform facilitates the transfer of nutrients and products. The processcan be successfully scaled up from laboratory scale to 10L bioreactor, thus demonstrating the feasibility of theS. cerevisiaeA2 strainto be used as a novel strain to produce ethanol
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