Mosquito acquisition of the virus likely occurs during a blood meal; after uptake, the virus replicates and is transmitted to a reservoir animal at the next blood meal (32). Isolation of the virus or of anti-Zika virus antibodies from various nonhuman primates and other wild and domestic animals suggests multiple animal reservoirs (33). One study examined the kinetics of Zika virus infectivity in Ae. aegypti mosquitoes by using blood-feeding membranes (27); viral content was high on the day of feeding (inoculation), decreased to undetectable levels through day 10, increased by day 15, and remained high on days 20–60. These findings suggest an incubation period in mosquitoes of ≈10 days.