Thefeedingbehavioranddigestivephysiologyoftheseacucumber,Apostichopusjaponicusarenotwellunder-
stood.Abetterunderstandingmayprovideusefulinformationforthedevelopmentoftheaquacultureofthisspe-
cies.Inthisarticlethetentaclelocomotion,feedingrhythms,ingestionrate(IR),fecesproductionrate(FPR)and
digestiveenzymeactivitieswerestudiedinthreesizegroups(small,mediumandlarge)ofseacucumberundera
12hlight/12hdarkcycle.Frame-by-framevideoanalysisrevealedthatallsizegroupshadsimilarfeedingstrat-
egiesusingagraspingmotiontopickupsedimentparticles.Thetentacleinsertionratesofthelargesizegroup
weresignificantlyfasterthanthoseofthesmallandmedium-sizedgroups(Pb0.05).Feedingactivitiesinvesti-
gatedbychargecoupleddevicecameraswithinfraredsystemsindicatedthatallsizegroupsofseacucumber
werenocturnalandtheirfeedingpeaksoccurredat02:00–04:00.