The DH values of amylose–fatty acid complexes decreased with increasing carbon chain length, indicating the amount of complexes formed between amylose and fatty acids followed the order of LA > MA > PA. Interestingly, the DH values of
amylose–fatty acid complexes in retrograded starch samples were higher as compared with gelatinized NWSE–fatty acid samples on immediate rescanning, indicating that more amylose–fatty acid complexes formed during storage at 4 C. Taken together, we can conclude that the amount of amylose–fatty acid complex formed is affected by the types of fatty acids and storage conditions. In addition to the melting peak of fatty acids, no peak was observed for WWS–fatty acid samples, indicating that complexes formed between WWS and fatty acids were too little to be detected by DSC even on longer storage.