methods
--LimulusLimuluslysatelysatefor for endotoxinendotoxin*Gram *––bacteria produce endotoxinendotoxin, which , consists of lipopolysaccharidelipopolysaccharide(LPS)(LPS)
**LimulusLimulusamoebocyteamoebocytelasatelasate(LAL) obtain (from the blood cell of the horseshoe crabfrom crab
*LAL is sensitive to *endotoxinendotoxin
*adding of food aliquots of food suspension *to a lysatelysateportionportion
*incubation, *endotoxinendotoxincauses the gel causes formationformation**E. coliE. colicells