Apuleia leiocarpa is a tree found in Caatinga that has great value in the timber industry. Lectins are
carbohydrate-binding proteins with several biotechnological applications. This study shows the isolation,
characterization, and antibacterial activity of A. leiocarpa seed lectin (ApulSL). The lectin was chromatographically
isolated from a crude extract (in 150 mM NaCl) by using a chitin column. ApulSL adsorbed to
the matrix and was eluted using 1.0 M acetic acid. Native ApulSL was characterized as a 55.8-kDa acidic
protein. SDS-PAGE showed three polypeptide bands, whereas two-dimensional electrophoresis revealed
four spots. The peptides detected by MALDI TOF/TOF did not show sufficient homology (