For the germination tests, four replicates of 50 seeds of each
primed and unprimed seeds were imbibed on two layers of blotter
paper in 9-cm-diameter Petri dishes at 10, 15 and 25 ◦C incubation.
Primed and unprimed Seeds were surface sterilized by soaking in
0.4% sodium hypochlorite (v/v) for 60 seconds (s), followed by three
60 s rinses in the sterile distilled, deionized (dd) water, and then
dried at room temperature.