An important increase in resistant starch (RS) level was obtained in the spaghetti with the addition of banana flour. The control sample showed the lowest (1.11%) value of RS, whilst the spaghetti containing 45% of banana flour possessed the highest value (12.42%). This is related with the high RS content of banana flour (42.5%). It was reported that unripe banana is the natural product with the highest RS content, because RS values of 57.2 and 47.3% were determined in unripe banana flour using two different methods (Faisant et al., 1995). The value of RS present in the control sample was lower than that reported for other spaghettis made with 100% semolina of 2.92% (Goñi and Valentín-Gamazo(2003)and 3.85% (Torres et al., 2007a; Torres et al., 2007b); whilstRS values of the samples with added banana flour were higher thanthose reported in noodles with added banana starch (3–4.6%,Oso-rio-Díaz et al., 2008).