. DM intake was measured daily and live weight weekly. Ex vivo ConcanavalinA (ConA) stimulated cell proliferation of peripheral blood mononuclear cells (PBMC) was tested by MTT and alamar blue (AB) assay. Serum was analysed for clinical chemical parameters, ion (Mg, Ca and P) and REE concentrations.
The effects of LaCl3, CeCl3, NdCl3 and YCl3 on ConA-stimulated proliferation of PBMC were tested in vitro, using MTT and AB assay.
REE-citrate supplementation did affect DM intake, but not live weight gain, clinical chemical parameters, and ion concentrations significantly. In REE-300 group ex vivo proliferation of PBMC was significantly increased. In vitro ConA-stimulated proliferation decreased with rising REE-chloride concentrations. At least at the highest tested concentration (approximately 290M) the inhibition reached significance.
Proliferation of non-stimulated PBMC was not affected dose-dependently.
REE affect the proliferation of PBMC, thus an effect on the bovine immune system is possible. However, the great differences in effective doses in vitro and ex vivo (serum REE concentrations) might explain the
different results from the experiments.