Pharmaceutical and cosmetic industries have a great demand for A. vera material. A. vera is an ancient, traditional medicine, and in modern herbal industries, it occupies a key position, as it is used in almost all kinds of herbal preparations; therefore, it is considered as an ancient plant for modern herbal industries. Although this plant is used in the herbal industry, some of the genotypes of A. vera (sweet aloes) are edible and are consumed as a vegetable and to make curry and desserts. Due to wide use of aloe products as alternative medicines and dietary supplements and with the rapid expansion of the aloe product industries (herbal, pharmaceutical, and cosmetic), there is an increased demand for its leaves as raw material. Current production of leaves is insufficient to meet the industry demand. Thus, there is a need to undertake large-scale cultivation of selected genotype(s). Propagation of A. vera by conventional methods or by means of offshoots is slow; a single plant produces three to four offshoots every year. The presence of male sterility is also a barrier in rapid propagation [4]. The vegetative propagation of aloe is insufficient to meet the increasing demand. Thus, there is limited availability of standard clonal propagules for commercial planting of elite/selected genotype(s). Tissue culture and in vitro plant regeneration systems provide alternative production systems for mass propagation of the desired plant species. One of the most extensive uses of plant tissue culture techniques is rapid and large-scale multiplication of plants, where the natural propagation rate is not meeting the demand.