The final concentrations of each of the treatments using sodium acetate as a carbon source after five days of culture can be seen in Figure 1. The highest amount of biomass obtained was 3.4 g/L in the treatment with 20 mM acetate and 2.94 mM nitrate. Cultures with 20 mM acetate had higher biomass concentrations than other cultures; treatments with lower concentration of nitrogen (1.02 mM) had final concentrations of biomass under the control culture (without 2.94 mM acetate and nitrate). In general, the final biomass concentration was positively correlated with the initial concentration of nitrate, indicating the flattering effect of nitrogen in the growth reported in the literature.