However, it is difficult to quantify the
lethal effects in filamentous fungi, as the effects of freezing that damage
vegetative cells can vary greatly, in accordance with the phase of cell
growth, the conditions under which they are grown, exposure to
other types of stress before freezing and the presence or absence of
cryoprotectants in the medium (Gill, 2011).
However, it is difficult to quantify the
lethal effects in filamentous fungi, as the effects of freezing that damage
vegetative cells can vary greatly, in accordance with the phase of cell
growth, the conditions under which they are grown, exposure to
other types of stress before freezing and the presence or absence of
cryoprotectants in the medium (Gill, 2011).
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