The following review briefly summarizes all current and former methods of body fluid identification and focuses on the new developments in forensic science during the last 5–6 years. It discusses both significant improvements in conventional bioanalytical methods and developments of novel approaches. The review evaluates the advantages and disadvantages each method presents, and it concludes with the discussion of new biospectroscopy methods under development that offer non-destructive confirmatory identification of body fluid traces immediately at the crime scene [15] and [16]. It is important to emphasize that these new biospectroscopy techniques are still being developed and are not yet available. We believe that with further testing, these novel methods will be able to deliver results in a simple and automatic fashion at a crime scene that will be acceptable for court testimony. For specific and in-depth details about a particular test, including former tests, tests currently in use, and newly developed tests, it is best to consult the individual articles and books referenced throughout the review.