The in vivo produced AMF single species cultures were isolated from pot cultures ofPlantago lanceolata.Theywere maintained on an initially sterilized (2×15 min at 121 °C, with a 12-h interval) substrate composed of a mixture of TerraGreen (Agsorb 8/16 LVM-GA, Chicago, IL, USA), fine quartz, and coarse quartz (2:2:1). The plants were grown in sunbags (Sigma-Aldrich, USA), under greenhouse conditions (set at min 20 °C during winter) with natural light intensity. Cultures were kept for at least 5 months with several thousands of spores produced within this period.