by 34 cycles at 94 °C for 30 s, annealing for 30 s at the primer's annealing
temperature, extension at 72 °C for 1 min 20 s and a final extension at
72 °C for 5 min.
The PCR product was run on a 1% agarose gel stained with ethidium
bromide.
PCR primers for 3 SSR markers of A. occidentale were used to amplify
different regions of the plant genome, as described by Croxford et al.
(2006) (Table 2).