Result and Discussion
Isolation of Trichoderma spp. from the soil samples
Seven isolates of Trichoderma spp. were collected from rhizosphere soils around tomato plants in Kamptee, Wardhamna, campus and PKV agricultural fields of Nagpur, India. Among these, Trichoderma virens and Trichoderma hamatum isolates were from Kamptee field. After incubation for 3 days, colonies of Trichoderma spp. on PDA and Malt Extract Agar (MA) at 28 °C ±1 °C were developed and became dark green at the center.