The theoretical and experimental studies were carried out, which resulted in the development of
acetylcholinesterase-based conductometric biosensor for determination of aflatoxin B1. We established
a linear range of substrate measurement (0.25–1 mM) and optimal concentration of acetylcholine for
inhibitory analysis (1 mM). The sensitivity of developed biosensor to aflatoxin B1 was tested, the calibration
curve was plotted for the dependence of residual activity of the acetylcholinesterase-based
bioselective element on the toxin concentration. Detection limit of aflatoxin B1 was 0.05 g/ml. It was
shown that the developed biosensor was characterized by high signal reproducibility of direct determination
of the main substrate as well as of the inhibitory analysis of aflatoxin B1 (the error of detection
was no more than 7%).