Sprouting of seeds was observed after 7th day of inoculation and 95% of sterile seedlings were obtained. In vitro plantlet regeneration from shoot explants and callus induction in MS medium supplemented with various concentrations of auxins and cytokinins are reported for Capsicum chinense Jacq. (Naga King Chilli). In vitro regeneration of Capsicum species is reported to be difficult [6,11] from nodal and shoot tips explants loosely arranged pale green friable calli were obtained after 30 days of inoculation.