Reaction procedure
Soybean oil was mixed with DMC followed by addition of the enzyme and the
schematic diagram for the enzymatic transesterification of vegetable oil with DMC is
shown Fig. 1 [15,17,25]. Reactions were carried out in 20 ml glass vials with working
volumes of 10 ml. Reactions ran for 48 h. Temperature and rpm were maintained
using a water batch circulator (Jeio-Tech, Korea).