For E. coli and S. aureus, 50 g sample was
placed in BPW and the suspension was serially
diluted in BPW to 10-3. One-ml was plated onto
PetriflimTM E. coli/ Coliform count plate (3M) and
PetrifilmTM Staph Express Count plate (3M) for
determination of E. coli and S. aureus, respectively.
The inoculated petrifilms were incubated at 370C for
24 hours. The samples were considered positive when
there was the presence of Salmonella in 25 g raw meat
and/or E. coli and S. aureus counts exceeded 100
colonies/g.