Elbeltagy et al. (2001) found that the acetylene reduction
activities of O. officinalis W0012 seedlings inoculated with
diazotrophic Herbaspirillum sp. strain B501gfp showed ethylene
production at a rate of 71.3±22.4 nmol h−1 g wet
weight−1. Nitrogen fixationwas also detected in leaf consortia,
which indicates the ability of EDB to fix nitrogen in plant
leaves (Prakamhang et al. 2009). Nitrogen fixation activity in
the endophytic stage did differ from nitrogen fixation in the
free-living stage.