The role of the defense enzymes peroxidase, polyphenol oxidase and β-1.3-glucanase, and the
proteins and chlorophyll content was investigated. The experiments in vitro and in greenhouse had been constituted by
extracts from liquid medium culture filtrate of P. sanguineus. Water, acibenzolar-S-methyl (ASM: 75 mg i.a./L) and
fungicide (azoxystrobin: 40 mg i.a./L) were the control treatments. In vitro, the extract of liquid medium culture filtrate
did not inhibit the mycelial growth, sporulation and conidia germination of P. griseola. Severity was reduced in 82%
and 49% in greenhouse and field respectively. The activities of peroxidase, polyphenol oxidase and the content of
proteins and chlorophylls had an increment in plants treated with extracts. These results indicate the potential of P.
sanguineus liquid medium culture filtrate for alternative control of the angular leaf spot in bean plants [