There are different types of tobacco available in Bangladeshi
markets all year round. The dried tobacco leaves samples werecollected from one of the biggest tobacco industry at Dhaka
Metropolitan City, Bangladesh in April 2009. Five fresh tobacco
leaves samples were collected from southern district of
Kushtia after harvest. The leaves samples were washed by
tap and de-ionized water to remove dusts and any other foreign
particles. After collection, the sample was kept in a polyethylene
bag with aluminum foil protected cover and stored in
refrigerator to avoid any deterioration. After harvesting, the
apples are stored at 2 C.