Discussion
The present study demonstrates for the first time, isolation of FLA from routine use water sources in critical units of a hospital in India. Most of the studies regarding isolation of FLA from water sources and biofilms of hospitals are reported from Europe, Iran, Egypt and Brazil.[4,12-15] The patients in BMT unit, HDU, TICU and HU constitute a group of high risk population who are more susceptible to diseases by any pathogen. The water sources of these units were screened for FLA which may be pathogenic themselves or may act as vehicle for carrying bugs especially drug resistant micro organisms which may cause nosocomial infections. Our study has demonstrated presence of FLA (morphological forms of trophozoites and cysts) in 14% of water and swab samples. Four of these samples were identified as Acanthamoeba species on