Heilongjiang Provinces of China. Certified Reference Materials
(CRMs) containing 1% genetically modified Bt11 maize, 2% genetically
modified Bt176 maize and 2% genetically modified Roundup
ready soya were used as positive controls. The results showed that
zSSIIb (Zea maize starch synthase isoform) and lectin (lectin is a
major protein in soybean) genes were detected in all samples
and CRMs. While Bt-11, Bt-176, 35S-CTP4, CP4EPSPS, CaMV35S
and NOS genes (transgenes) were only detected in the GMO CRMs
by using portable pyrosequencer; the corresponding DNA fragment
does not exist in the samples from market. The results are shown
in Table 2.