Estimation the Aloin purity by using the High Performance:
Liquid Chromatography [HPLC] technique:
The work was carried out at the Ministry of Science and Technology Department of Materials
Research, Dr. Fadel Mohsen. For certification of the previous characterization and for determination the degree of Aloin sample partial purity and comparison with standard one this analysis was done. The result of this test showed there was close compatible curves for the sample with that of standard one in the shape, but slightly different in the retention time, The partial purified Aloin sample appeared at [1.462] minutes while standard Aloin at [1.465] minutes as shown in Figure-1, from these results, the concentration ratio of Aloin tested sample was calculated, which was reached 91.84%, and these results indicated the high purity of the tested sample.