. Briefly, bead
beating was used to homogenize individual copepods, and
extraction was accomplished by coupling Zymo Direct-zol
reagents (Zymo Research, CA, USA) with phenol:chloroform
purification steps. Two SYBR green – (Fast SYBR1; ABI Step
One Plus-Life Technologies) based quantitative polymerase chain
reactions (qPCR), one for each isoform,