2. Materials and methods
2.1. Preparation of raw bone particles
Waste bones were collected from local slaughter houses, butch- ers, and restaurants. The bones were manually cleaned from meat pieces, repeatedly washed, and subsequently boiled in distilled water for 4 h in order to remove fats. The cleaning process was repeated 4 times. Thereafter, the clean bones were dried at 100 °C for 8 h. The dried bones were crushed, milled, and sieved into 4 different particle size ranges which were (>0.075), (0.075– 0.30), (0.3–1.18) and (1.18–2.34) mm for the powder, small, med- ium, and large size-particles, respectively.
2. Materials and methods2.1. Preparation of raw bone particlesWaste bones were collected from local slaughter houses, butch- ers, and restaurants. The bones were manually cleaned from meat pieces, repeatedly washed, and subsequently boiled in distilled water for 4 h in order to remove fats. The cleaning process was repeated 4 times. Thereafter, the clean bones were dried at 100 °C for 8 h. The dried bones were crushed, milled, and sieved into 4 different particle size ranges which were (>0.075), (0.075– 0.30), (0.3–1.18) and (1.18–2.34) mm for the powder, small, med- ium, and large size-particles, respectively.
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