The formulation of laccase presented in this study leads torecover 35% of the initial enzyme activity used in process for theconsidered best formulation. This value might seems to be lowerbut actually it is quite high considering that there are several lay-ers of protein [15] at the interface oil–water and it is reasonableto expect that only the most external layer would contribute forthe enzyme activity of the emulsion. The extra minutes obtainedas half-life time of the enzyme under ultrasound (Table 2) makesthe all difference in effectiveness for textile bleaching (see databelow in Table 3 after bleaching). The thermal and pH stabilitywere expressed as the half-life time for free and laccase micro-emulsion using 30 min of exposure (Table 2). Half-life time wascalculated from exponential decay (graphical abstract) and the time