,-glucosidase (ft-d-glucoside glucohydrolase,
EC 3.2.1.21) was extracted from the coleoptiles of 5- to 6-
day-old maize seedlings with 50 millimolar sodium acetate, pH
5.0. The pH of the extract was adjusted to 4.6, and most of the
contaminating proteins were cryoprecipitated at 0°C for 24 hours.